Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: Pathogenicity, serotype, and host distribution of Y. enterocolitica strains

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: Isolation location and biotype distribution of Y. enterocolitica strains

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques: Isolation

Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: Sequence pattern number of each housekeeping gene for each species

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques: Sequencing

Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: Neighbor-joining tree of 1,015 isolates, housekeeping genes, and concatenated sequences (topology graph). Besides three pathogenic Yersinia species, Y. similis and the Korean group are colored according to the description by Laukkanen-Ninios et al. (19). **, ST187 was the only sequence type shared by pathogenic and nonpathogenic Y. enterocolitica strains. The nonpathogenic strain was a biotype 1A ail+ strain. *, Two particular nonpathogenic, ystB+ Y. enterocolitica strains, ST184, clustered next to ystB-negative strain STs (ST181 to ST183), and ST185, a biotype 1A ail+ strain, clustering next to pathogenic Y. enterocolitica STs, indicating its potential pathogenicity. s.s., sensu stricto.

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques: Sequencing

Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: Minimum spanning tree, colored by species, of three pathogenic Yersinia species before (A) and after (B) importing the UCC database sequences and combining them with 352 strains collected by our laboratory. Pathogenic Yersinia species fell into two distinct clusters; one included Y. pseudotuberculosis and Y. pestis, and the other involved pathogenic and nonpathogenic Y. enterocolitica strains. Y. pseudotuberculosis STs were numerous and scattering, Y. enterocolitica was relatively conserved, especially within pathogenic strains, and Y. pestis was the least diverse. The Arabic numerals show ST assignments. The circle sizes are directly proportional to the numbers of isolates. The numbers of locus differences are represented by bold lines (1 locus), plain lines (2 loci), black dotted lines (3 loci), dark-gray dotted lines (4 loci), and light-gray dotted lines (>5 loci). The colored shaded areas around the circles represent single-locus variants (SLVs).

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: ystB distribution in nonpathogenic Y. enterocolitica strains. Within the nonpathogenic Y. enterocolitica strains, ystB-negative strains (ST181 to ST183) had seven radically different loci compared to ystB+ strains. Two particular ystB+ strains (ST184 and ST185) had no identical loci with either ystB-negative strains or other ystB+ strains.

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica , Yersinia pseudotuberculosis , and Yersinia pestis Based on Multilocus Sequence Typing

doi: 10.1128/JCM.02185-13

Figure Lengend Snippet: Minimum spanning tree of pathogenic Y. enterocolitica strains, colored by serotype. A strong specificity pattern for O serotypes within the pathogenic strains was demonstrated. ST187 and ST186 include most pathogenic strains. Nonpathogenic Y. enterocolitica STs are more heterogeneous, presenting genetic diversity through evolution. Same-serotype strains of pathogenic and nonpathogenic Y. enterocolitica strains were distributed in separate branches of the tree.

Article Snippet: Commercial serotype identification test kits for Y. enterocolitica were purchased from Denka Seiken Co., Ltd., Japan, and the Institute of Chinese Biomedicine.

Techniques: